【摘要】 枯草芽孢杆菌Ｂ３（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）在ＮＢ，Ｋ＇Ｂ，ＬＢ，ＹＴ，Ｍ９和ＳＧ等６种培养液中产生抗菌物质的量有显著差别，以在ＮＢ中产量最大。以ＮＢ作培养基时，Ｂ３在ｐＨ５～９、温度为２０～４０℃范围内均可产生抗菌物质，而又以ｐＨ７．０和３０℃最适。定期取样测定表明，Ｂ３在培养１２～４８ｈ内产生抗菌物质逐渐增多。不同碳氮源测试结果表明，以终浓度为０．１％的葡萄糖、０．２％的甘露醇或０．２％的可溶性淀粉作碳源，０．１％的硫酸铵、氯化铵、硝酸铵或半胱氨酸作氮源有利于Ｂ３抗菌物质产生。更多还原

【Abstract】 There is a significant variation in quantity of antagonistic substances (AS) produced by B3(B. subtilis),according to media used (NB, K’B, LB, YT, M9 and SG), with the maximum in NB medium. In NB medium with pH5. 0-9. 0, B3 can produce AS at 20-40℃, while pH7. 0 and 30℃ are the best conditions. Antagonistic activity of B3 cell-free culture filtrates obtained from 12 h to 48 h is gradually increased. Out of 9 kinds of carbon and 6 nitrogen sources, glucose(0. 1 % ), mannitol (0. 2 % ) or soluble starch (0. 2 % ) as sole carbon source, (NH4 )2SO4 (0. 1 % ),NH4Cl (0. 1 % ), NH4NO3 (0. 1 % ) or cysteine (0. 1 % ) as sole nitrogen sources are suitable for production of B3 AS.更多还原