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油菜素内酯对湿地松苗的生理作用

THE PHYSIOLOGICAL EFFECTS OF BRASSINOLIDE(BR) IN PINUS ELLIOTTII ENGELM. SEEDLINGS

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【作者】 汪安琳高强陈裕菊

【Author】 Wang Anlin; Gao Qiang; Chen Yuju (College of Forest Resources and Environment Nanjing Forestry University Nanjing 210037)

【机构】 南京林业大学森林资源与环境学院

【摘要】 早春经0.5mg·L-1和1.0mg·L-1油菜素内酯(BR)水溶液淋灌处理的湿地松一年生苗,在5、6月份生长高峰期间表现促进高生长。在9、10月份促进茎的加粗生长。12月下旬测定生物量,BR处理苗比对照增加了17.39%~26.37%。从6月至12月的几个生理指标测定表明,针叶的叶绿素含量一直保持较高水平。可利用总糖含量除生长高峰时期降低以外,也始终高于对照。在炎热、干旱的7、8月份针叶内SOD活性明显比对照增强,而MDA合量低于对照。1月初测得BR处理苗针叶的可溶性糖含量比对照提高了16%~31%。

【Abstract】 One-year-old Pinus elliottii seedlings were treated with 0. 5 and 1. 0 mg·L-1 BR on March 30, 1994 in the field. From April 1 to December 21, the height growth of seedlings were measured monthly.According to the conditions of seasonal growth,the contents of chlorophyll,total sugar and malondialdehyde (MDA), and the activity of superoxide dismutase(SOD) were tested.The diameter growth and the biological products of seedlings have also been determined.The results showed that from 7/1 to 12/22 the data of BR treated leaves maintained higner level of chlorophyll content than the control.For exemple, in July,seedlings were under drought and high temperature stress,the chlorophyll contents(mg·g-1 FW. )in CK and BR 0. 5, 1. 0 mg· L-1 treated ones were 0. 348, 0. 451, 0. 448, and in December, under low temperature,the contents of chlorophyll were 0. 28,0. 36, 0. 44 respectively.In general, the coments of total sugar kept a high level in MR treated seedlings, except in June, the month of height growth, the total sugar was decreased. From 8/1 to 12/22 (tests for five months), the average contents of total auger (% of FW) in CK and BR 0. 5,1. 0 mg·L-1 treated leaves were 8. 58, 9. 91. 9. 84.In the months of 7, 8. 9, under the condition of drought and high temperature, the average activities of SOD (inhibition of NBT reduction %) in CK and BR 0. 5. 1. 0 mg·L-1 treated leaves were 47. 29, 52. 81, 56. 78. The average contents of MDA (nmol·g-1 FW) were 13. 6, 12. 83, 11. 26. There is an obvious inverse correlation between SOD activity and MDA content.BR 0. 5 and 1. 0 mg·L-1 could promote both the height and diameter growth of seedlings. From Aprill 1 to December 21 the net growth in heigh(cm) were 11. 58, 4. 1; and the net diameter growth (cm) were 0. 65, 0. 59. The dry weight (g) of seedlings (average of 15 seedlings) were 28. 27, 26. 26. These data were much higher than CK: 2. 85 cm, 0. 49 cm and 22. 37 g.This research confirms that BR(0. 5 and 1. 0 mg·L-1) can improve foe resistance and tolerance of pine seedlings under stress of drought and high or low temperature. BR is not only able to enhance the height and diameter growth of seedlings. but also promote the growth of branches and the root system.

【基金】 国家自然科学基金
  • 【文献出处】 南京林业大学学报 ,JOURNAL OF NANJING FORESTRY UNVERSITY , 编辑部邮箱 ,1995年04期
  • 【分类号】S791.246.05
  • 【被引频次】45
  • 【下载频次】119
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