【摘要】 本文采用MTT比色分析法对比观察了从绿豆中分离提纯的苯丙氨酸氨解酶（PAL）对小鼠白血病L₁₂₁₀细胞和人白血病K₅₆₂细胞的作用。结果表明:（1）PAL对白血病细胞有明显的抑制作用,2.8μ/ml的酶作用48小时后抑制率分别达62.1％和82.6％,与空白对照组相比有显著性差异（P<0.01）。（2）随酶剂量的增加,抑制效果明显增强,同样作用48小时,0.70μ/ml的酶其抑制率分别为5.2％和14.1％,当酶增加为3.5μ/ml,可分别达77.1％和85.8％（P<0.001）。更多还原

【Abstract】 The effects of Phenylalanine Ammonia-Lyase (PAL) purified from mung bean on Mouse leukemia cell line L1210 and Human leukemia cell line K562 were observed in Vitro with MTT colorimetric assay. The results showed that (1)Inhibitory effect of PAL was evident on leukemia cell line. The rates of Inhibiting L1210 and K562 were 62.1% and 82.6% respectively for 48 houre when 2. 8u/ml of PAL was used (P<0. 01). (2)The inhibitory effects became more marked with the increase of PAL concentration. When concentration of PAL was 0. 7u/ml and 3. 5u/ml the inhibition rates were 5. 2%, 14.1% and 77.1%, 85. 8% respectively (P<0. 001).更多还原