【摘要】 对实验性缺血的大鼠心肌切片进行免疫组化染色，检测肌酸激酶。结果显示，在心肌缺血４０ｍｉｎ后，着色开始呈现酶活性减少的变化，并随缺血时间延长而更加明显。实验证实肌酸激酶的免疫组化染色，是一种诊断早期心肌缺血的特异性强、敏感性高的重要辅助方法。更多还原

【Abstract】 The immunohistochemical stain for estimating creatine kinase was made in the experimental myocardial ischemic sections of rats, the results showed that the color change of enzymatic activitic decrease occurred after 40 min ischemia, and this phenomena became more prominent accompany by extension of ischemic time. This experimemt proved that the creatine kinase PAP staining method is an important supplemental method and has higher specificity and sensitivity for early myocardial ischemia.更多还原