【摘要】 作者从ｐＳＰ７３中分离出２２１ｈｐ多克隆区整合至ＰＭＷ１１９。从ｐＳＫ６和ｐＳＫ１６中分离出葡糖基转移酶基因ｇｔｆＢ和ｇｔｆＣ．并整合至这一重组质粒中，分别形成ｐＴＦ４１和ｐＴＦ４２。从ｐＶＡ８３８中分离出链球菌复制子并整合至ｐＴＦ４１和ｐＴＦ４２的适当部位．分别构成重组质粒ｐＺＢ１和ｐＺＢ２。ｐＺＢ１和ｐＺＢ２为分别含ｇｔｆＢ或ｇｔｆＣ基因的链球菌－大肠杆菌穿梭质粒。更多还原

【Abstract】 Abstract The 221 base pair multiclonal site from pSP73 was isolated and ligated to a low copy number plasmid,PMW 119. The resultant plasmid, namely, pTF37,was then digested and Ligated with glueosyltransferase genes from S. mutans MT8148. The chimeric plasmid pTF41 (carrying gtfB ).pTF42 (carrying gtfC) were digested and ligated wwith a Streptococcus replicon isolated.from pVA838. The chimeric plasmid were designated pZB1 (carrying gtfB) and pZB2 (carrying gtfC). By this strategy,We successfully constructed the Streptococcus-Escherichia colt shutter vector pZB1 and pZB2 containing gtf genes.更多还原