【摘要】 一株美洲粘虫ＧＶ（增效品系）在东方粘虫幼虫上进行增殖，所获粘虫ＧＶ（ＰｕＧＶ－Ｐｓ）对东方粘虫ＮＰＶ（ＰｓＮＰＶ）、棉铃虫ＮＰＶ（ＨａＮＰＶ）和黄地老虎ＮＰＶ（ＡｓＮＰＶ）进行增效试验。实验结果证明ＰｕＧＶ－Ｐｓ对三种ＮＰＶ都有明显的增效作用，其中以对ＰＳＮＰＶ为最强，增效率达５５％－８５％对ＨａＮＰＶ为３０％－８０％；而对ＡｓＮＰＶ只有１５％－３５％。用凝胶过滤技术从ＰＳＧＶ－Ｐｓ中分离增效因子ＰｕＳＦ－Ｐｓ，其对ＰｓＮＰＶ和ＡｓＮＰｙ的增效作用亦同样明显，２５０μｇ的ＰｕＳＦ－Ｐｓ可以提高ＰｓＮＰＶ的感染能力达８０％。经Ｓｅｐｈａｄｅｒ－１５０柱（１．６×９０）和使用四种标准蛋白（细胞色素Ｃ、胃蛋白酶、牛血清清蛋白和乳酸脱氢酶）测得ＰＳＳＦ－Ｐｓ的分子量为１６００００左右。更多还原

【Abstract】 We have propagated Pteudaletis unipuncta granulosis virus (synergistic strain) in larvae of Pseudaletis separata and have conducted quantitative evaluation of the enhancement of the P. unipuncta granulosis virus-Ps (PuGV-Ps) on the NPVs of P. separata (Ps), Heliothis armigera (Ha) and Agrotis segetum (As). The results show the PuGV-Ps enhanced PsNPV, HaNPV and AsNPV infections in their original host insects. The high proportions (55 %-85 %, 30 %-80% ) of enhancement were observed in the PsNPV and HaNPV assays, and the median one (15%-35%) was obtained in the AsNPV-PsGV system. A synergistic factor (PuSF-Ps) has been isolated from the PuGV-Ps system using gel filtration technique. When PsNPV polyhedra were mixed with 250 ng PuSF-Ps, the value of enhancement increased by about 80%. The molecular weight of the synergistic factor was determined with Sephadex150 column (1.6cm × 90cm) to be about 160k.更多还原