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磷化氢、二氧化碳混合气体对腐食酪螨成螨的生物学效应
SOME BIOCHEMICAL ASPECTS OF PHOSPHINE IN COMBINATION WITH CARBON DIOXIDE AGAINST THE ADULTS OF TYROPHAGUS PUTRESCENTIA (SCHRANK)(ASTIGMATA: ACARIDAE)
【摘要】 本文研究结果表明:腐食酪螨Tyrophagusputrescentiae(Schrank)成螨在0%、8%、16%(容积比)CO2气体中耗氧量随CO2浓度的增加而增加,当在32%、64%CO2气体中,该成螨的耗氧量反倒低于其在正常大气中的耗氧量。在0%、8%、16%、32%、64%CO2与0.05mg/LPH3混合气体中该成螨对PH3的吸收量分别为1.11±0.92、1.79±0.56、5.14±1.13、7.60±1.80、8.08±0.85μg/hr’g,在同一CO2浓度条件下试螨对PH3的吸收量在高浓度PH3(0.45mg/L)中明显大于在低浓度PH3(0.05mg/L)中,但PH3吸收量的增加倍数远远低于PH3浓度的增加倍数。PH3对该螨过氧化氢酶的抑制体内酶高于离体酶,细胞色素c氧化酶受PH3抑制则相反。被PH3抑制的过氧化氢酶和细胞色素C氧化酶活性恢复时间分别为二周和一周。本文还对PH3的可能杀螨机理及CO2在此过程中的作用进行了讨论
【Abstract】 This study shows that there is an increase in oxygen consumption of the adults of Tyrophagus putrescentiae (Schrank) when the concentration of ambient carbon dioxide rises from 0% to 8%and to 16%, but further increase in the carbon dioxide concentration up to 32% and 64% causes the decrease of the oxygen consumption of the tested mites. In mixed fumigation with 0%, 8%, 16%, 32%,64% CO2 and 0.5mg/l PH3, the uptake of phosphine is 1.11 ± 0.92, 1.79 ± 0.56,5.14 ±1.13, 7.60 ± 1.80, 8.08 ± 0.85μg/hr·g respectively. The uptake of phosphine was found to increase with increase of phosphine concentration at the same concentration of carbon dioxide. However, the uptake did not increase markedly when the concentration of phosphine (0.05mg/l. 0.45mg/l) was multiplied. The results of in vitro and in vivo experiments led to the conclusions that phosphine inhibits the activity of cytochrome C oxidase and catalase, but a lower inhibitory effect on cytochrome C oxidase can be detected in in vivo experiments and the inhibition can be kept 1 week after exposure to the fumigant. The catalase was inhibited markedly in tested mites poisoned with phosphine and the inhibition was kept 2 weeks after exposure to the fumigant. In addition, the mode of action of phosphine and the role of CO2 are discussed.
【Key words】 Tyrophagus putrescentiae (Schrank); phosphine; cytochrome C oxidase.catalase; oxygen consumption;
- 【文献出处】 昆虫学报 ,ACTA ENTOMOLOGICA SINICA , 编辑部邮箱 ,1995年01期
- 【分类号】S482.5
- 【被引频次】12
- 【下载频次】62