Purification of β-adrenergic receptors from Beijing duck erythrocyte plasma membranes and their reconstitution with Gs and adenylate cyclase on asolectin liposomes

【摘要】 <正> Catecholamines such as adrenaline, norepinephrine and isoproterenol regulate a wide variety of physiological responses via their specific binding to adrenergic receptors located in the plasma membrane. Adrenergic receptors have been divided into two major types, α and β. Binding of ligands to β-adrenergic receptors (β-AR) first triggers the activation of stimulatory GTP-binding protein (Gs). The activated Gs then interacts with更多还原