【摘要】 应用核酸杂交技术（ｄｏｔ－ｂｌｏｔ）分析了癌基因。－ｍｙｃ、 Ｎ－ｒａｓ和抑癌基因 ｐ５３在骨髓增生异常综合征（ＭＤＳ）和急非淋白血病（ＡＮＬＬ）中的扩增情况。结果发现，有 ３ 例（３３％）的 ＭＤＳ存在 ｐ５３抑癌基因的 ２倍扩增，本实验中 ｐ５３抑癌基因的扩增发生在 ＭＤＳ的较晚期阶段； １例 ＡＮＬＬ中存在癌基因 ｃ－ｍｙｃ和抑癌基因 ｐ５３的共扩增，且发现扩增与 ＭＤＳ的分型及 ＡＮＬＬ的临床病情有一定的相关性。更多还原

【Abstract】 Using dot-blot hybridization, the authors studied the amplification of c-myc, N-ras oncogenes and p53 tumor suppressor gene in marrow specimens from 9 cases of myelodysplastic syndrome(MDS), 11 cases of acute nonlymphoblastic leukemia(ANLL), 9 cases of ANLL-CR and 11 cases of other blood diseases. p53 tumor suppressor gene was found to be amplified in 3 MDS, 3 ANLL and I ANLL-CR patients. c-myc oncogene was found to be amplified in 18. 2 percent of ANLL patients. None of the MDS patients was found to have amplified c-myc oncogene. Furthermore, none of the patients had amplified N-ras oncogene. Relating these results with clinical features, it was found that the amplification of p53 tumor suppressor gene took place at a later stage of MDS.更多还原