【摘要】 以ＤＥＮ制成实验性肝癌大鼠，同时连续１２周分别给予肝癌大鼠干扰素α－２ｂ（ＩＦＮ）、卡介苗（ＢＣＧ）或两者联用，对照组给予生理盐水。于第１２周和１６周分离培养各组动物肝枯否细胞（ＫＣ）和血单核细胞（ＭＣ），并分别与人肝癌细胞联合培养，用￣３Ｈ－ＴｄＲ掺入法测定ＫＣ和ＭＣ对肝癌细胞的抑增殖作用。分离培养第１８周和２２周对照组肝癌大鼠的ＫＣ经体外ＩＦＮ、ＢＣＧ或两者联用后，同上测定ＫＣ对肝癌细胞的抑增殖作用。结果显示：１．肝癌大鼠的ＫＣ对肝癌细胞增殖仍具有一定的自然抑制作用，ＫＣ的作用明显强于ＭＣ；２．上述免疫刺激剂的在体应用或体外对ＫＣ的直接作用，均以ＩＦＮ和ＢＣＧ联用增强ＫＣ的抑肝癌细胞作用最强，最强者可增强５倍以上；３．单用免疫刺激剂者，ＢＣＧ的效果较ＩＦＮ强。讨论分析了联用免疫刺激剂增强ＫＣ抗肝癌最佳效果的机制及其实用意义。更多还原

【Abstract】 Hepatoma was induced in rats by administration of diethylnitrosamine(DEN).The rats with hepatoma were treated respectively by IFNa2b,BCG,or combination of both,the controls were not treated with any biological immune stimulants(BIS).The Kupffer cells(KC)and Monocytes(MC)were isolated from the rats(at 12 th and 16 th week),and cocultured respectively with human SMMC-7 7 2 1 hepatoma cells,their cytostatic activity against human SMMC-7 7 2 1 hepatoma cells were assaied through measuring3 H-TdR incorporation into tumor cell DNA. The KC were isolated from control rats and treated with BIS in vitro,and their cytostatic activity against human SMMC-7721 hepatoma cells were also measured.The results showed that KC and MC of rats with hepatoma still presented latent natural cytostatic activity against hepatoma cells,KC was more effective than MC. The stimulative effects of combination of BIS on KC and MC were more effective than that of single stimulant treatment in vivo or in vitro,BCG was more effective than IFN,The mechanism and its significance for the cytostatic effect of KC enhanced by BCG ,IFNα-2b and than combination on hepatoma cells were briefly discussed.更多还原