【摘要】 用免疫组织化学ABC法结合葡萄糖氧化酶-DAB-硫酸镍铵(GDN)增强显色技术,对大鼠肠系膜上动脉和静脉及其分支中NPY免疫反应神经纤维的形态和分布进行了研究,并用交点计数法统计了其密度。结果发现,NPY免疫反应神经纤维的密度随肠系膜上动脉和静脉分支级别的增加而增加;肠系膜上动脉和静脉中相应分支级别上的NPY免疫反应神经纤维的密度没有显著差异。更多还原

【Abstract】 In this work free-floating staining with immunohistochemical ABC method combined nickel enhancement technique was used to visualize NPY-immunoreactive nerve fibres in the superior mesenter-ic artery (SMA) and vein (SMV) and their branches of rat. The density of these vessels was measured by intersection-counting method. We demonstrated that the density of NPY-immunoreactive nerve fibres increased with the levels of branches either in the arteries or in the veins. Moreover, the density between the accompanied arteries and veins do not show significance.更多还原