【摘要】 本文用细胞培养方法，观察脑血管内皮细胞粘附分子（ＩＣＡＭ－１）的表达和内皮细胞与血液单核细胞的粘附作用。结果显示，１０ｕ／ｍｌＴＮＦ－α即可使内皮细胞ＩＣＡＭ－１表达增加，自发性高血压大鼠（ＳＨＲ）增加的程度大于正常血压大鼠（ＷＫＹ）。干扰素（ＩＦＮ）和白细胞介素１（ＩＬ－１）也可使ＩＣＡＭ－１表达增加。高浓度细胞因子均使ＳＨＲ和ＷＫＹ内皮细胞ＩＣＡＭ－１表达增加，但二者之间无明显差别。经ＬＰＳ或ＩＬ－１和ＴＮＦ－α刺激的ＳＨＲ内皮细胞与单核细胞的粘附率明显高于ＷＫＹ。结果证实，ＴＮＦ－α，ＩＬ－１和ＩＦＮ可使ＳＨＲ和ＷＫＹ脑血管内皮细胞ＩＣＡＭ－１表达和单核细胞粘附增加，两组动物细胞相比，ＳＨＲ内皮细胞ＩＣＡＭ－１表达及粘附率增加较多，提示ＳＨＲ脑血管内皮细胞可能对细胞因子的敏感性升高。更多还原

【Abstract】 ell adhesion plays a very important role in many pathological processes. The expression ofICAM-1 on cultured cerebrovascular endothelial cells and the adhesion reaction of the endothelialcells with monocytes in vitro were observed in this experiment. The results showed that 10u/mlTNF-α increased more expression of ICAM-1 in SHR endothelial cells than that in WKY. Highconcentration of cytokines increased the ICAM-1 to a higher level in both SHR and WKY, but nosignificant difference between them. Comparison of adhesion reaction, the ability of freshly iso-lated peripheral blood monocyte to adhere to cerebrovascular endothelial cells treated with LPS orIL-1 and TNF-α was significantly greater in cell cultures from SHR than from WKY. These datademonstrate the increased expression of ICAM-1 on endothelial cells from SHR. Also, the in-creased snesitivity of endothelial cell cultures to cytokines was related to hypertension.更多还原