Correlation of metabolic changes of infected paulownia tissue culture with PWB-MLO pathogenic mechanism

【摘要】 <正> Paulownia sp.tissue culture plantlets infected with paulownia witches’ broom mycoplasma-likeorganism （PWB-MLO） expressed a typical witches’ broom symptom.The results determined by transmissionelectron microscopy and DAPI staining fluorescence microscopy showed that the severity of the symptom wasdirectly related with MLO concentration in the sieve tubes of the phloem.The results of the analysis ofendogenous hormones and phenolic compounds by HPLC revealed that in all cases,the free IAA content ofthe diseased plant apparently lower than that of the healthy,and the free IAA concentration had strikinglypositive correlation with catechol concentration changes （r=0.90,n= 11）.The changes of cytokinin （Z +ZR,iPA）,GA₃ and ABA were shown less relation with MLO infection.The differences of peroxidase andisoenzyme,IAA oxidase and phenylanine ammonia lyase （PAL） between the extract of infected plants andthat of uninfected as well as different effects of NAA and catechol additions on MS media could further re-flect the nature of the relationship and the interaction among MLOs,catechol and the free IAA concentration.更多还原

【Abstract】 Paulownia sp.tissue culture plantlets infected with paulownia witches’ broom mycoplasma-like organism （PWB-MLO） expressed a typical witches’ broom symptom.The results determined by transmission electron microscopy and DAPI staining fluorescence microscopy showed that the severity of the symptom was directly related with MLO concentration in the sieve tubes of the phloem.The results of the analysis of endogenous hormones and phenolic compounds by HPLC revealed that in all cases,the free IAA content of the diseased plant apparently lower than that of the healthy,and the free IAA concentration had strikingly positive correlation with catechol concentration changes （r=0.90,n= 11）.The changes of cytokinin （Z +ZR, iPA）,GA₃ and ABA were shown less relation with MLO infection.The differences of peroxidase and isoenzyme,IAA oxidase and phenylanine ammonia lyase （PAL） between the extract of infected plants and that of uninfected as well as different effects of NAA and catechol additions on MS media could further re- flect the nature of the relationship and the interaction among MLOs,catechol and the free IAA concentration.更多还原