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大肠癌细胞核DNA含量的流式细胞分析

Analysis of Nuclear DNA Content in Colonic Cancer

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【作者】 吴瑾宁小明张安田殷积美刘爱春

【Author】 Wu Jin et al(Department of Internal Medicine,The Third Affiliated Hospital)

【机构】 哈尔滨市医院血液科

【摘要】 我们应用流式细胞术对35例大肠癌术后标本进行了DNA含量分析。结果表明,异倍体肿瘤占62.9%(22/35),二倍体肿瘤占37.1%(13/35),与以往文献报道相符;二倍体肿瘤的DNA含量明显低于异倍体肿瘤;二倍体肿瘤的G0/G1期细胞数明显高于异倍体肿瘤,而S、G2M期细胞数则明显低于异倍体肿瘤。这说明,DNA的倍体水平可作为大肠癌诊断及判断预后的一项辅助指标。

【Abstract】 The DNA contents in 35 paraffin-embedded samples of colonic cancer were analyzed using flow cytometry.The rate of DNA non-diploid tumor was 62.9%(22/35) and was unanimous to the pre-reports.The DNA contents in DN A diploid tumors were tess than that of DNA nondiploid tumors.The cell numbers in Go/Giphases of DNA diploid tumors were significantly greater than that of DNA non-diploid tumors,while the cell num-bers in S,G2M phases of the fermer were less than that of the later(P<0.05).These findings sugguest that DNA ploidy be considered as an objective parameter of colonic cancer for diagnosis and the measuring of cell scatter in every phases has certain value for clinical features.

【关键词】 大肠癌流式细胞术DNA倍体类型
【Key words】 Colonic cancerFlow cytometryDNA ploidy
  • 【文献出处】 哈尔滨医科大学学报 ,JOURNAL OF HARBIN MEDICAL UNIVERSITY , 编辑部邮箱 ,1995年02期
  • 【分类号】R735.340.2
  • 【被引频次】2
  • 【下载频次】19
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