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我国主要致病钩端螺旋体DNA与OmpL1基因同源性的研究
Homology Study of Leptospires in China with the OmpL1 Gene
【摘要】 用 ̄(32)P标记的OmpL1基因片段与我国18株钩体进行Southern杂交分析,此片段与非致病钩体PatocI株、伊利尼细丝体3055株无杂交信号;问号钩体中,除爪哇群、塔拉索夫群、曼耗群和明尼群的4株钩体外,黄疸出血群、犬群、拜伦群、致热群、秋季群、澳洲群、波摩那群、流感伤寒群、七日热群、巴达维亚群、赛罗群各群钩体参考株DNA均有杂交信号。
【Abstract】 Southern hybridization analysis withthe OmpL 1 gene was performed. The results of prob-ing EcoRI-restricted genomic DNA from 1 8 st rains inChina showed that the homology fragments of OmpL1gene were presented in 1 2 pathogenic Leptospirastrains:sero-group Icterohaemorrhagiae(2 strains),Canicola,Ballum,Pyrogenes,Autumnalis,Australis,Pomona,Grip-potyphosa,Hebdomadis,Bataviae,andSejroe,but they were not presented in the non-pathogenic Leptospira biflexa strain Patoc I,Leptone-ma illini strain 3055 and pathogenic I,eptospiraSerogroup Javanica,Tarassovi,Manhao and Mini.
- 【文献出处】 华西医科大学学报 ,JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES , 编辑部邮箱 ,1995年03期
- 【分类号】R377.5
- 【被引频次】9
- 【下载频次】16