【摘要】 采用经层析方法提纯的减蛋综合征１９７６（ＥＤＳ＇７６）病毒免疫ＢＬＡＢ／ｃ小鼠。最后一次加强免疫后第３ｄ制备脾脏细胞，然后用５０％的聚乙二醇（ＰＥＧ，分子量４０００）融合脾脏细胞和ＳＰ２／０骨髓瘤细胞。通过选择培养、特异性抗体检测、筛选和克隆，最后制备出５株分泌抗体的细胞株，命名为Ｍ２，Ｍ３，Ｍ１０，Ｍ１４和Ｍ１５。微量血凝抑制试验（ＨＩ）证明Ｍ１５单克隆抗体具有血凝抑制活性，ＨＩ价为６（ｌｏｇ２）。对单克隆抗体进行测定显示５株均对ＥＤＳ＇７６病毒有很强的特异性，与传染性支气管炎病毒（ＩＢＶ）、传染性喉气管炎病毒（ＩＬＴＶ）、甲型流感病毒（ＩｎｆｌｕｅｎｚａＡｖｉ－ｒｕｓ）、鸡胚致死性孤儿病毒（ＣＥＬＯ）、新城疫病毒（ＮＤＶ）和传染性囊病病毒（ＩＢＤＶ）无交叉反应。更多还原

【Abstract】 BLAB/C mice were immunized with chromatographically purified EDS’76 virusV205 strain,and splenocytes were prepared three days after the final boosterimmunization. Fusion of lymphocyte and myeloma SP2/0 cell line was carriedout with the fusogen 50% polyethylene glycol(M.W. 4 000).Five antibodysecreting cel lines,named M2,M3,M10,M14 and M15,were finally obtained afterselective cultivation,specific antibody detection and cloning.It was shown thatM15 monoclonal antibody had haemagglutination inhibition activity with a titerof 6(log2)in microplate HI test.All five McAbs were determined to be stronglyspecific to EDS’76 virus with no reaction to IBV,ILTV,influenza A virus,CELOvirus,NDV and IBDV.更多还原