【摘要】 应用感染貉细小病毒（RPV）的72h猫肾传代细胞(F₈₁)制做细胞抗原片。利用超迷离心结合蔗糖密度梯度离心法提纯RPV病毒作为免疫用抗原,制备了豚鼠抗RPV高免血消。在酶标SPA染色法的基础上测得高免血清最佳工作范围为160^×、320^×、640^×;PPA的最佳工作效价为40^×;被检样品与标准阳性血清作用最佳温度为37℃,时间为60min。确立了酶标SPA抑制染色法的试验程序。并对有关试剂保存有效期进行了试验测定。更多还原

【Abstract】 In the present paper.the cell antigen slids was prepared Usingfeline Kindey cells infected by raccoon parvorius (RPV)for 72 hours.RPV,grown in feline kindey cells,was parified through the sucrose densitygradient hypervelocity centrifugation. Hypcrimmune sera against RPV Wereobtaincd fron guinea pigs which were inoculated with purified RPVcombined with Freurd adjuvant. PPA inhibition staing was established forthe detection of RPV.更多还原