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维生素B＿2产生菌的原生质体制备及再生
Preparation and Regeneration of Protoplasts from Vitamin B2 Producer Eremothecium aohbyii
【摘要】 用１．０％纤维素酶＋０．５％蜗牛酶的混合酶液酶解培养２６～３２小时的维生素Ｂ＿２产生菌阿氏假囊酵母（Ｅｒｅｍｏｔｈｅｃｉｕｍａｏｈｂｙｉｉ）菌丝体，可以得到大量原生质体，最佳酶解时间为１．５小时。原生质体再生率为０．３５％～０．６７％。阿氏假囊酵母在原生质体再生过程中具有较高的突变率，以维生素Ｂ＿２产量为指标的正突变率为１２．１４％。
【Abstract】 Using a combined enzyme system containing 1.0% cellulase（ONOZUKAR-10）plus 0.5 % snail digestase,high yield of protoplasts from Eremothecium aohbyiimycelia which had grown for 26-32 hours was obtained.The regeneration frequency were0.35%-0.67%. Many mutants appeared in the regeneration strains on solid medium. Posi-tive mutation ratio was 12.14% by determining the yield of Vitamin B2.
- 【文献出处】 河北大学学报(自然科学版) ,JOURNAL OF HEBEI UNIVERSITY(NATURAL SCIENCE) , 编辑部邮箱 ,1995年01期
- 【分类号】Q93
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