【摘要】 对Ｐ５３阳性的８例肝细胞性肝癌（ＨＣＣ）广西科学，１９９５，２（１）：５５～５７）中的７例的Ｐ５３基因进行ＤＮＡ单键构象多态性分析（ＳＳＣＰ）和ＰＣＲ扩增物直接测序。结果，７例均在Ｐ５３第７外显子第２４９编码区的３号位发现异常。１例在２４９编码区的第３碱基微小丧失引起乱码突变；另６例在此位点同时出现突变的Ｔ和Ｃ带，成为的多态性突变形式。外显子５、６、８和９没有异常。估计除黄曲霉毒素Ｂ１外可能有别的致癌因素协同作用造成此种多态性。认为南宁地区是继中国江苏启东和莫桑比克后的第３个ＨＣＣＰ５３基因２４９编码区有集中突变热点的地区。更多还原

【Abstract】 P53 gene DNA of 7 cases of 8 hepatocellular carcinomas(HCC) with positive P53 mutation (Guangxi Sciences, 1995, 2 (1): 55～57) were detected by and proceeded for PCR-SSCP analysis and DNA direct sequencing. 7 HCCs showed a single point abnormality at position 3 of exon 7codon 249. One showed a microdelection at position 3 of codon 249 leading to a frame shift, othersix showed two bands for mutants T & C at the third base of codon 249, leading to a polymorphic mutation form. No other mutation was in exon 5, 6, 8 and 9. There may be more other factors besides aftaloxin B1 for these polymorphic mutations. Nanning prefecture could be confirmed as a third area in the world with mutational hot-spot at canon 249 in the P53 gene in HCC.更多还原