【摘要】 通过对武乡海棠（Malus honanensis Rehd.）优良矮砧S₁₈、S₂₀两个单系的0.2mm微茎尖脱毒组织培养技术研究,解决了微茎尖切割、接种、分化及生根移栽等一系列技术难题,获得了S₂₀、S₁₈一批脱毒试管苗及移栽壮苗.分化数分别达2.0和3.6,生根率达76.1%和90.0%,平均根长2cm以上,平均根数5条,移栽成活率达78%.更多还原

【Abstract】 Two tenth milimeter microstem of M. honanensis Rehd. superior apple dwarf rootstock S18 and S20 were collected during shoot growth period, sterilized, and cultured in the MS medium. A Series of trouble occurred during cultivation, including the excision and inoculation of the microstem, shoot differentiation, rooting, devitrification, and transplantating, were overcomed. The virus-free planlets were obtained which were identified by indicative plants. The rate of proliferation reached 2. 0-3. 6; percentage of rooting reached 76. 1%-90. 2%; root number was 5 per planlet, mean root length was 2 cm; survival percentage of transplantation reached 78%.更多还原