【摘要】 利用国产大孔硅胶作基质合成了疏水填料．按照高效疏水作用色谱法，采用梯度洗脱方式分离了６种标准蛋白及唾液中α－淀粉酶和基因工程生产的γ－干扰素．柱子不可逆吸附小、被试验的α－淀粉酶和溶菌酶活性几乎定量被回收．应用合成的色谱填料研究了洗脱剂中盐浓度和温度对蛋白质保留行为的影响，论证了合成填料的色谱属性．更多还原

【Abstract】 A HIC support has been synthesized by using the domestic silica with major pore Diameter. The mixture of six standard proteins,α-Amylase in saliva and rIFN-γ from E. Coli.were separated through gradient elution by high-performance hydrophobic interaction chromatograply respectively.The chromatographic column has less irreversible adsorption,α-Amylase and lysozyme used in this study are almost quantitatively recovered on thiscolumn. The effects of salt concentration in eluent and temperature on retention behaviours of proteins were investigated with packing prepared,and the chromatographic attribution of the support has been demonstrated.更多还原