【摘要】 电导法测脲酶活性朱元保，颜流水，何双娥（湖南大学化学化工系，长沙，４１００８２）关键词脲酶活性，电导法，米氏常数，活化能脲酶广泛存在于豆类中，脲酶活性已成为豆乳质量控制指标之一，目前测脲酶活性的常用方法有比色法、氨电极法和酸碱滴定法。本文根据脲酶催化...更多还原

【Abstract】 A rapid and simple conductometric method has been estabilished for the assay of urease activity.Experimental results showed that the method has the advantages of high sensitivity and wide linear range of 0. 02 to 0. 03U/ mL,and does not need other reagents except substrate.The Michaelis constant and activation energy of the enzymatical reaction were also determined,which are in good agreement with previously reported values.Furthermore,the proposed method had been applied to assay urease content from swordbean and soybean with a RSD within 0.8% and a recovery of 95% to 105%.更多还原