【摘要】 <正>化学和生物发光是由化学反应产生的一种光辐射,不需要任何光源。又由于它们具有高灵敏度、宽线性范围和相对比较便宜的仪器等优点,因而在化学和生物传感器领域引起了广泛的兴趣。已用于H₂O₂、乳酸和胆固醇等多种生物活性物质的测定,但未见有金属离子传感器的报道。本文发展了一种新型的全固态模式的消耗型锰离子化学发光传感器。该传感器将除待测物外的所有化学发光反应试剂全部固定在阴离子交换树脂Amberlyst A-27上,于化学发光反应之前,将一定量化学发光试剂从固定化试剂柱上洗脱,与样品中的锰离子产生化学发光。已成功地应用于水样中痕量锰离子的测定。每个固定化试剂柱可连续使用100次以上。 1 实验部分 1.1 仪器和试剂 化学发光传感器由流动系统和检测系统两部分组成。其中流动系统主要由蠕动泵、六通阀、固定化试剂柱和流通池组成。检测系统由光电信增管、负高压、放大器和记录仪组成（图1）。更多还原

【Abstract】 A chemiluminescence sensor for Mn2+ combined with flow-injection system is presented in this paper. It was based on the new approach that all of the reagents involved in the chemiluminescence reaction were immobilized electrostatically on Amberlyst A-27 anion-ex-change resin. Mn2+ can be sensed directly by the reaction with Luminol and IO4-, which were eluted by Na3PO4 from the immobilization column prior to the chemiluminescence reaction. The linear range for Mn2+ is 2. 0×10-9-1.0×10-5g/mL. The detection limit is 1.0×10-9 g/mL. The column with immobilized chemiluminescence reagents in the resin can be used for 100 times. The sensor has been applied to the determination of Mn2+ in water samples.更多还原