【摘要】 本文研究了在ＣＴＭＡＢ存在下，用钙黄绿素蓝荧光法测定食品中的微量锌（Ⅱ）的实验条件。在本实验条件下，Ｚｎ（Ⅱ）与钙黄绿素蓝生成１：１的配合物使钙黄绿素蓝的荧光增强，最大激发波长和最大发射波长分别为３５３ｎｍ和４４０ｎｍ。锌量的线性范围为０．０１７～０．７０μｇ／１０ｍＬ，检测限为１．７ｎｇ／ｍＬ，标准曲线的中间部分的相对标准偏差为１．５６％。方法用于测定脱脂奶粉、大米粉、含锌营养食盐中的微量锌（Ⅱ），相对标准偏差分别为２．５９％、４．６０％和０．４９％。更多还原

【Abstract】 In this work,a new spectrofluorimetricmethod for the deterrnination of zinc(Ⅱ)in foodstuff with calcein blue(CB)was devel-oped. The formation of CB-Zn(Ⅱ)complexleading to the enhanceinent of fluorescenceintensity of CB was applied to the determi-nation of Zn(Ⅱ).The addition of CTMAB(cetyltrimethyammonium bromide)to the solution of CB-Zn(Ⅱ)complex produces anincrease of fluorescence intensity of CB andan increase of stability of the Zn(Ⅱ)-CBcomplex. The maximum excitation and e-mission wavelengths of CB reagent blankare 332 and 440nm and those of the CB-Zn(Ⅱ)complex are 353 and 440nm,respec-tively, The concentration range for a linearrelationship is 0. 017～0.70μg/10mL andthe detection limit is 1. 7ng/mL for Zn(Ⅱ).The analytical results indicate a precision of1. 56%at the middle region of the calibra-tion curve,This method is simple and selec-tive for Zn(Ⅱ)and it has been applied tothe determination of Zn (Ⅱ)in foodstuffwith satisfactory results.更多还原