【摘要】 本文报道了新荧光试剂３－对甲苯基－５－（２’－羧基苯偶氨）绕丹宁（ＰＭ－ＰＲＡＣＰ）的合成。用元素分析、红外光谱、核磁共振氢谱和质谱确证了其结构，研究了其荧光性质。在ｐＨ５．６时，该试剂与Ｃｕ￣（２＋）形成稳定的荧光螯合物，且在λ＿（ｅｘ）／λ＿（ｅｍ）＝３０５ｎｍ／４０５ｎｍ产生强烈荧光。其荧光强度与Ｃｕ￣（２＋）的浓度在０～１．６μｇ／２５ｍＬ范围内呈线性关系，检测限为５×１０￣（－４）μｇ／ｇ。已用于痕量铜（Ⅱ）的荧光分光光度法测定。测定了蚕茧、蚕丝和丝绸中的痕量铜，结果满意。更多还原

【Abstract】 This paper reports the synthesis of 3-p-tolyl-5-（2’-carboxyphenylazo）rhodanine（PMPRACP ）.5.6g o-aminobenzoic acidwas made into diazonium salt , then coupledwith 5. 6g 3-p-methylrhodanine in ammoniawater,giving yellow needle crystals,m,p.164～166℃,The product has been con-firmed by elemental analysis , IR , HNMRand MS.A fluorescence analytical methodfor Cu（Ⅱ）was developed with this reagent.Toa 25 mL volumetric flaskwese added 1mL1.0μg/mL Cu（Ⅱ） working solution,0. 5mL1×10￣（-4）mol.L￣（-1）PMPRACP solution and2mL pH5.66 buffer solution,the mixtusewas thenheated for 30 min to form the fluo-rescent chelate, and measured at λ_ex/λ_em=305nm/405nm, There is a linear relationshipbetween the fluorescence intensity and con-centration of Cu￣（2+） in the range of 0-1.6μg/25mL, The detection limit is 5 × 1 0￣（-4） μg/g.The reagent has been used in fluorimetricdetermination of trace Cu in cocoon, naturalsilk and silk cloth with satisfactory results.更多还原