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木毒蛾颗粒体病毒的研究
Researches into the Granulose Virus of Lymantria xylina Swinhoe
【摘要】 木毒蛾组织病变观察,颗粒体病毒能侵染虫体的脂肪体、中肠及马氏管等组织,但不侵染气管.在透射电镜下,颗粒体呈圆形卵圆形,大小为320~830nm×230~570um。超薄切片观察,卵圆形包涵体中具单杆病毒粒子.室内外感染试验表明,用颗粒体病毒粗提液1.2×106~1.2×109个/mL感染4~6龄幼虫,死亡率达81.8%~100%,杀虫力很强,是一种有价值的微生物杀虫剂.
【Abstract】 In the May of 1991, a new insect virus was discovered in ajing Village, Xiapu County, Fujian Prvince,and identified as Lymantria china Swinhoe granulose virus. This is the first report of the virus in the world. The body of larvae become nat-long after infection, hanging on the branch like the shape of the letter V,the colour of body become dark, the skin get tough, the tissue in body become brown like paste. Through dissecting tissue in the laboratory, the results indicate that the virsu infect fat tissue first and make the fat tissue expanded, and then infect midgut and malpighian tubes, but not infect windpipe. The virus multiply in the cell mucleus. The infected tissue break up and the virus particles proliferated throughout body when the larvae dead. The granulose virus is oval or circular shape under electron microscope, the size is 520-830um ×230~570um, the surface looks smooth. The mortalities of 4~6-aged larvae treated with granulose virus suspensions with concentrations of 1. 2 × 109, 1. 2 × 108, 1. 2 × 107 and 1. 2 × 106 (particles/mL)reach respectively to 100%, 97. 7 % 93. 3 % and 83. 3 %. Therefore, this virus suspension is a desirable microbiological insecticide.
- 【文献出处】 福建林学院学报 ,JOURNAL OF FUJIAN COLLEGE OF FORESTRY , 编辑部邮箱 ,1995年03期
- 【分类号】S763.729.3
- 【被引频次】9
- 【下载频次】54