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外源基因在球形芽孢杆菌中的转化
Elimination of plasmid and transformation into Bacillus sphaericus
【摘要】 为了扩大芽孢杆菌属的受体系统范围,对球形芽孢杆菌进行了质粒消除,并用含有溶葡球菌酶基因的质粒pE194cop6-1转化。结果表明,该酶基因在球形芽孢杆菌中获得了稳定、高效表达。
【Abstract】 In order to expend the host system,plasmid in B. sphaericus was eliminated and then was transformed with plasmid pE194 cop 6-1 containing lysostaphin gene into this strain. It was showed that the lysostaphin gene could express stably in high level in Bacillus sphaericus.
- 【文献出处】 复旦学报(自然科学版) , 编辑部邮箱 ,1995年04期
- 【分类号】Q939.124
- 【被引频次】2
- 【下载频次】79