【摘要】 本文采用聚合酶链反应(PCR)技术,通过扩增鼠疫菌9.5kb质粒pla基因上一个456bp片段,用于鼠疫菌的快速诊断。对4株鼠疫菌参考株PCR扩增后,琼脂糖凝胶电泳分析均呈现单—DNA扩增区带,其分子量大小与预期结果相符。对13株非鼠疫菌参考株作平行实验,PCR扩增后未见扩增区带。其检测的敏感性为10个cfu。全部实验可在4h内完成。对鼠疫菌的快速诊断和分子流行病学调查具有重要意义。更多还原

【Abstract】 We developed apolymerase chain reaction assay by which a 456 bp of pla gene fragment carried by 9. 5Kb plasmid of Y. pestis was amplified. The PCR method has advantage over other available methods for the rapid diagnosis of Y. pestis. 4 Y. pestis strains were detected by the amplification of PCR, the amplified bands through agarose gel electrophoresis showed to tally with the desired result. 12 other strains were examinated at the same condition, and no amplified fragments appeared. The sensitivity of this method is 10 cfu. Only 4h was taken to complish the all test. The application of this method has great significance in the rapid diagnosis of Y. pestis and investigation of molecular epidemiology.更多还原