【摘要】 将诱导出的胚性愈伤组织采用：①逐级提高盐浓度进行胁迫筛选；②直接转入高浓度胁迫筛选；③先用羟脯氨酸（Ｈｙｐ）筛选后再转入高浓度盐中进行胁迫筛选，结果表明三种筛选方法其效果为③＞②＞①，即先用Ｈｙｐ筛选再进一步盐胁迫筛选效果最好。并对选出的耐盐细胞进行了生长量测定和生理生化特性的研究，表明耐盐细胞系脯氨酸含量不仅稳定，而且远高于对照，Ｋ￣＋／Ｎａ￣＋也大于对照，ＳＯＤ活性增加，小分子ＳＯＤ含量增加，本文并对有关问题进行了讨论。更多还原

【Abstract】 The embryonic callus were selected by the following method:(1)cultured on medium ofwhich the NaCl concentration was increased step by step,(2)directly cultured on medium withhigh NaCl concentration,(3)first cultured on medium containing hydroxyproline,then on medi-um containing high concentration of NaCl.The results showed that the last method is the bestand the second is better than the first,indirect selection is more practicable than direct.Thephysiological and biochemical characters of NaCl resistant callus were also studied.Notable differ-ences between the NaCl resistant callus and the control had been found.Free proline content,K￣+/Na￣+ and superoxide dismutase activity were much higher in NaCl resistant callus than con-trol.In addition,electrophoretic analysis revealed that the low molecular weight superoxide dis-mutase was enbanced.In the end,some relevant questions were discussed.更多还原