【摘要】 沙蒿种子灭菌后，接入ＭＳ无激素培养基得到无菌苗。取２０天无菌苗子叶切成小块，接入含２，４一Ｄ的ＭＳ培养基诱导愈伤组织，并在同样培养基上进行继代增殖。愈伤组织在含６－ＢＡ、ＮＡＡ的ＭＳ培养基上分化得到芽。将芽转至含ＩＡＡ的１／２ＭＳ培养基上形成根，从而得到完整植株。同时对影响沙蒿组织培养的一些因素进行了研究。结果表明培养基中２，４一Ｄ含量（０．５一２ｍｇ／Ｌ）越高，沙蒿愈伤组织生长越快，而褐化发生时间却提前；活性碳对抑制沙蒿愈伤组织褐化有明显作用，与水解乳蛋白相比，水解酪蛋白对沙蒿愈伤组织的生长更为有利；ＩＡＡ对沙蒿苗根的分化是必需的。更多还原

【Abstract】 After sterilizing,seeds of sand sagebrush (Artemisia sphaerocephala Krasch) were plantedon MS medium without phytohormine, Cotyledons of twenty day old seedlings were sliced andplaced on MS medium containing 2,4一Dto induce calli. Calli were stibcultured on the samemedium。 Shoots differentiated from callus on the MS medium containing 6- BA, When the shootswere transferred on the MS medium with IAA, roots could induced. The influence of some fac-tors on tissue culture of this specise was studied. It suggested that the higher concentration of2,4一Din the medium favoured the callus formation CH exhibited better effect for callus growingthan LH。 Callus browning was efficiently prevented by adding activated charcoal in the medium。Some other reducing agents,i.g。, citric acid and vitamin C also retarded browning to a certain extent。更多还原