【摘要】 用聚合酶链反应（ＰＣＲ）获得抗－ＨＢｓ单克隆抗体Ｆｖ段轻、重链基因，经连接后形成一条单链含轻、重链的Ｆｖ基因（ＳｃＦｖ），并在重组－噬菌体－抗体系统（ＲＰＡＳ）中获得表达，用酶联免疫吸附法（ＥＬＩＳＡ）证明表达的Ｆｖ段具有特异性抗原结合活性。更多还原

【Abstract】 mmunoglobin Fv genes of anti-HBs McAb were amplified by PCR from a hybridoma cell line secreting anti-HBs monoclonal antibody.The heavy chain and light chain were linked to become single chain Fv gene fragment and were expressed by recombinant phage antibody system(RPAS).The specific antigen binding activity of the Fvexpressed was demonstrated by enzyme linked immunoassay.更多还原