【摘要】 在用毛细管电泳分离蛋白质时，为消除毛细管壁对蛋白质的吸附，以静态涂渍工艺制备了γ－缩甘油醚基三甲氧基硅烷及环氧基聚合物涂层毛细管柱．在ｐＨ３－５范围内，涂层有效地降低了管壁对蛋白质的吸附作用和电渗流．然而在更高ｐＨ值下，仍然存在着峰形畸变和柱效降低的现象．但实验表明，在ｐＨ＜５时，环氧基聚合物涂层具有较高的稳定性和良好的分析重复性．更多还原

【Abstract】 Fused-silica capillaries used in capillary zone electrophoresis were statically coated with γ- glycidoxypropyltrimethoxysilane and epoxy polymer in order to suppress wall adsorption in the separation of proteins. It has been shown that a significant decrease in adsorption was obtained and eletroosmotic flow was the diminished in the pH range 3-5. However with higher pH values, appreciable peak deformation and decreases in the resolving power were observed. Under pH 5, the epoxy polymer coating was shown to be quite stable and exhibited reproducible separations from run-to-run and day-to-day over a period of time.更多还原