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钙对花粉萌发的启动产效应和对花粉管生长的调节作用

Initiating Effects of Calcium on Pollen Germination and Its Regulative Role in Pollen Tube GroWth

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【作者】 龚明杨中汉曹宗巽

【Author】 GONG Ming YANG Zhonghan CAO Zongxun(T H Tsao)(Life Science College,Peking University,Beijing 10087)

【机构】 北京大学生命科学院

【摘要】 本文以云南松和烟草为材料,研究了钙对花粉萌发和花粉管生长的效应。结果表明花粉萌发介质中高浓度的钙(10 ̄(-1)~10 ̄(-1)2mol/L)显著抑制了云南松和烟草的花粉萌发和花粉管生长,而较低浓度的钙或缺钙(10 ̄(-3)~0mol/L)对花粉萌发和花粉管生长无明显影响。测定萌发过程中花粉钙含量变化的结果表明,花粉水合阶段和萌发早期有一急剧的钙释放高峰。在培养介质中加入钙螫合剂EGTA抑制了花粉的萌发,但这种抑制可被随后加入的钙所逆转。钙通道阻塞剂Vera-pamil和Co ̄(2+)也能抑制或阻止花粉萌发,但这种抑制主要发生在花粉萌发的早期。此外,在同样浓度的钙载离子体A_(23187)的处理下,花粉管生长比花粉萌发过程更为敏感。上述结果暗示着钙可能作为花粉萌发的启动者(Initiator)和花粉管生长的调节者(Regulator)来启动花粉萌发和调节花粉管生长。

【Abstract】 The initiating effects of Ca ̄(2+)on pollen germination and its regulative role in pollen tube growth were investigated in Pinus yunnanensis and Nicotiana tabacum. High concentration of Ca ̄(2-)(10 ̄(-1)~IO ̄(-2)mol/L)in the medium caused severe inhibition of pollen germination and tube growth,but lower concentration of Ca ̄(2+)or Ca ̄(2+)-deficiency(10 ̄(-3~0mol/L)had little effect on pollen ger-mination and tube growth. Measurement of Ca content in germinating pollen showed that there was a significant Ca ̄(+2) release from pollen during the hydration and early germination phase. How-ever,Ca ̄(2+)specific chelating agent EGTA led to the inhibition of pollen germination,and this inhibi-tion could be eliminated by re-adding excess Ca ̄(2+)to the medium containing EGTA. Ca ̄(2+)channel blockers verapamil and Co ̄(2+)also caused the inhibition or failure of pollen germination, In addition,Ca ̄(2+)ionophore A23187 impeded pollen germination and tube growth. Unlike veraparmil and Co ̄(2+)treatments,pollen tube growth was more sensitive to A23187 than pollen germination. These results suggested that Ca ̄(2+)is indispensible for pollen germination and tube growth although exogenous addition of Ca ̄(2+)to the medium is not necessary. The uptake of Ca ̄(2+)occurred mainly in early germi-nation phase, and Ca ̄(2+)could act as an initiator of pollen germination and also as a regulator of pollen tube growth.

  • 【文献出处】 北京大学学报(自然科学版) ,Acta Scicentiarum Naturalum Universitis Pekinesis , 编辑部邮箱 ,1995年02期
  • 【分类号】Q945
  • 【被引频次】93
  • 【下载频次】615
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