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中国河南西峡晚白垩世恐龙蛋化石基因分离及序列分析
DNA Ioslation and Sequence Analysis of Dinosaur DNA from Cretaceous Dinosaur Egg in Xixia Henan,ChinaLI Yi AN Chengca i
【摘要】 从河南省西峡晚白垩世一枚恐龙蛋化石内腔絮状内含物及外壳提取DNA样品,并用一段特异引物与6碱基随机引物进行PCR扩增、基因克隆和序列分析。结果表明用这对引物从蛋腔絮状内含物样品中扩增到一段150bp的DNA片段,推测氨基酸序列与EMBL库中已知蛋白质氨基酸序列进行了序列同源性分析,结果表明所克隆到的这段基因与非洲爪蟾表皮钙粘着蛋白(cad-herin)前体及脑细胞粘着蛋白、牛及人胎盘胞钙粘着蛋白在氨基酸水平有显著的序列同源性,分别为40.6%,37.5%,34.4%和36.4%。
【Abstract】 DNA was extracted from 70~80 million year old dinosaur egg excavat-ed from Cretaceous belt formation in Xixia Henan.China.The DNA samples from both outer shell and inner floccule contents were used as templates in polymerase chain reactions (PCR )with a specific primer and hexamers. With these two primers a l50 bp DNA fragment has been amplified , cloned and sequenced.Deduced amino acid sequence of this dinosaur DNA has been compared with known protein se-quences in EMBL Bank. The results showed that it has significant homology with Xenopus laevis epithelia precursor-Ep-cadherin(40.6%)and brain-cadherin(37.5%)、bovine placental cadherin(34.4%)and human placental cadherin(36;4%),respectively.
- 【文献出处】 北京大学学报(自然科学版) ,ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS , 编辑部邮箱 ,1995年02期
- 【分类号】Q911.27
- 【被引频次】4
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