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提高牛体外受精核移植胚胎发育率的研究

STUDY ON ENHANCEMENT OF DEVELOPMENT RATE OF NUCLEAR TRANSFERRED BOVINE EMBRYOS

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【作者】 廛洪武李荣凤旭日干

【Author】 CHAN Hongwu; LI Rongfeng; BOU Shorgan(the Research Centre for Laboralory Animal Science, Inner Mongolia University Hohhot 010021)

【机构】 内蒙古大学实验动物研究中心

【摘要】 本研究以体外成熟的、去核的牛卵母细胞为受体,以发育为8-32细胞期的牛体外受精胚胎为供体进行了核移植实验,着重探讨了激活方法和供体胚龄对移核胚发育的影响。结果表明:对去核受体进行三重激活(一,75V×80μs/2次电刺激;二,用含有10pg/ml放线菌酮的TCM199处理6小时;三,用5μmol/L的钙离子载体A23187处理5分钟)的移核胚囊胚的发育率为19.3%(22/114),高于一重激活(一)和二重激活(一和二)的发育率15.4%(16/104)和10.6%(13/123),但无显著差异;分别以体外受精后第2、3、4、5天的胚胎(8-32细胞)作为供体所获得的移核胚,均能够发育为囊胚,发育率为4.8%-11.7%,无明显差异。实验结果证明:本研究采用的方法对以体外成熟卵为受体,以体外受精胚为供体的牛细胞核移植是有效的;对去核受体卵子进行有效的激活处理,可以提高移核胚胎的发育率;供体胚的胚龄对移核胚的发育无明显影响。

【Abstract】 Nuclear transfer was carried out by using enucleated bovine oocytes(recipient cells),and blastomeres from in vitro fertilized embryos with 8-32 cells. The proportion of blastocysts from nuclear transferred embryos by three steps activation(electric pulsm→ cyclohexmidm→ Ionophore A23187) of the recipient cytoplasm was 19.3% (22/114), higher than 15.4%(16/104)from the two steps activation (electric pulse→cyclohexmide),and 10.6% (13/123) from one step activation (electric pulse),(p>0.05).The percentages of blastocysts after nuclear transfer with the donor cells from the in vitro fertilized embryos in different stages (day2-days) were 4.8%-11.7%. The results suggest that the treatment of recipients and the selection of donor in this study are effective. sufficient activation of the recipient oocytes is important,but selection of different stages of the in vitro fertilized embryos for donors appears no apparent influence on the results.

【关键词】 体外受精核移植
【Key words】 In vitro fertilization Nuclear transfer Cattle.
  • 【文献出处】 动物学杂志 ,CHINESE JOURNAL OF ZOOLOGY , 编辑部邮箱 ,1995年06期
  • 【分类号】Q954.4
  • 【被引频次】4
  • 【下载频次】65
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