【摘要】 本研究以体外成熟的、去核的牛卵母细胞为受体，以发育为８－３２细胞期的牛体外受精胚胎为供体进行了核移植实验，着重探讨了激活方法和供体胚龄对移核胚发育的影响。结果表明：对去核受体进行三重激活（一，７５Ｖ×８０μｓ／２次电刺激；二，用含有１０ｐｇ／ｍｌ放线菌酮的ＴＣＭ１９９处理６小时；三，用５μｍｏｌ／Ｌ的钙离子载体Ａ２３１８７处理５分钟）的移核胚囊胚的发育率为１９．３％（２２／１１４），高于一重激活（一）和二重激活（一和二）的发育率１５．４％（１６／１０４）和１０．６％（１３／１２３），但无显著差异；分别以体外受精后第２、３、４、５天的胚胎（８－３２细胞）作为供体所获得的移核胚，均能够发育为囊胚，发育率为４．８％－１１．７％，无明显差异。实验结果证明：本研究采用的方法对以体外成熟卵为受体，以体外受精胚为供体的牛细胞核移植是有效的；对去核受体卵子进行有效的激活处理，可以提高移核胚胎的发育率；供体胚的胚龄对移核胚的发育无明显影响。更多还原

【Abstract】 Nuclear transfer was carried out by using enucleated bovine oocytes(recipient cells),and blastomeres from in vitro fertilized embryos with 8-32 cells. The proportion of blastocysts from nuclear transferred embryos by three steps activation(electric pulsm→ cyclohexmidm→ Ionophore A23187) of the recipient cytoplasm was 19.3% (22/114), higher than 15.4%(16/104)from the two steps activation (electric pulse→cyclohexmide),and 10.6% (13/123) from one step activation (electric pulse),(p>0.05).The percentages of blastocysts after nuclear transfer with the donor cells from the in vitro fertilized embryos in different stages (day2-days) were 4.8%-11.7%. The results suggest that the treatment of recipients and the selection of donor in this study are effective. sufficient activation of the recipient oocytes is important,but selection of different stages of the in vitro fertilized embryos for donors appears no apparent influence on the results.更多还原