【摘要】 通过对比试验，筛选出含３．０％牛血清白蛋白的ＢＷＷ培养基和２·０ｍｏｌ／Ｌ最终浓度的二甲基 亚砜（ＤＭＳＯ）冷冻保护剂配制成冷冻液，将金黄仓鼠卵以０．３－１·０℃／ｍｉｎ的冷冻速率分别降温至 －４０℃和－８０℃，再浸入液氮中冷冻保存（３～３１天）。结果表明：冷冻降温速率以０·３℃－０·５℃／ｍｉｎ 为宜，降温到一８０℃浸入液氮的冻卵存活率（６３％）比－４０℃（４６％）的高。将解冻后存活卵去除 透明带，与人获能精子进行体外穿透试验，穿卵率达３４． ４％，与新鲜卵对照组（５０％）相比，无统 计学差异（Ｐ＞０·０５）；扫描电镜观察结果与压片光镜观察相吻合。更多还原

【Abstract】 A simple and feasible freezing method for the preservation of golden hamster oocytes was developed from a series of comparative experiments. Zona-intact oocytes slowly (0. 3℃/min) cooled in BWW medium containing 3. 0% BSA and 2. 0mol/L DMSO till -40℃ or - 80℃ and then were transfered them to liquid N2(- 196 ℃ ) for 3 -31 days. Af- ter thawing of frozen ova. the suvival percentage of the ova when slow cooling was terminated at - 80℃ (63%) was significantly greater than of the ova which was transferred at -40℃(46%) ; there were no obviously significantly differences in the periods time of preservation(3 -31 days) and the cooling rates (0. 3 ℃- １℃/min )and the dilution of DMSO (rapid or stepwize). After thawing, the survival zona-free golden hamster ova were penetrated by human capacitated spermatozoa SEM observation showed the process clearly. There was no difference between the penetration rates of human capacitated spermatozoa into frozen ova (34. 4% ) and that into the fresh (50%) collected oocytes. The resultes indicate that the survival of golden hamster ova stored at - 196 ℃offers a convenient means of supplying and transporting these eggs for the assessment of the fertilizing capacity of human spermatozoa.更多还原