【摘要】 应用抗重组Ｈ和Ｌ铁蛋白亚基单克隆抗体和间接ＥＬＩＳＡ检测方法，对３５例正常人、１００例肝癌患者血清铁蛋白Ｈ和Ｌ亚基进行定量分析，发现肝癌患者血清铁蛋白Ｈ和Ｌ亚基水平明显高于正常人（Ｐ＜Ｏ．０５）；两组血清铁蛋白Ｈ、Ｌ亚基比例相近（Ｐ＞０．０５）。肝癌患者血清铁蛋白Ｈ亚基水平的增高同时伴随着Ｌ亚基的增高（ｒ＝０．５，Ｐ＜０．０００５）。应用抗铁蛋白多抗检测了其中２０例正常人，６０例肝癌患者的血清铁蛋白浓度，其范围分别为８～３００μｇ／Ｌ（均值：１２７．２μｇ／Ｌ），６０～１８５μｇ／Ｌ（均值：５６４．４μｇ／Ｌ），与Ｌ亚基单抗检测结果相近（ｒ＝０．７，Ｐ＜０．０００５）。更多还原

【Abstract】 The serum ferritin H and L subunits were detected by ELISA in 35 blood donors and 100 patients with hepatocellular carcinoma(HCC) using monoclonal antibodies against H and L subunits(MoAb-H,MoAb-L). Serum ferritin H and L subunits levels in patients were higher than that in blood donors(P<0.05), but the H/L subunit ratio(<0. 2 in all sera) was nearly the same(P> 0.05). Sera with high level of H subunit always had increased L, there was a significant linear correlation between H and L subunits(r=0.5, P<0.001) These results suggest that HCC produce and release both basic and acidic isoferritin into circulation. 20 serum samples from 35 blood donors and 60 from 100 patients with HCC were measured for serum ferritin levels by ferritin diagnosis kit. Normal ranges for serum ferritin were 8-300ug/L (median, 127. 2μg/L), 60-1850μg/L for HCC(median, 564.4μg/L). The results are similar as that of using MoAb-L.更多还原