【摘要】 青杄（Ｐｉｃｅａ ｗ ｉｌｓｏｎｉｉＭａｓｔ．）胚性愈伤组织在改良５９ 附加２， ４－Ｄ及Ｋｔ各１ ｐｐｍ 的培养基上继代３ 年（每月继代１ 次），仍具有旺盛的增殖能力。在胚性愈伤组织转入１／２改良５９ 并附加ＡＢＡ １ ｐｐｍ 的分化培养基上，约３ 个月左右可分化出大量体细胞胚。体细胞胚分化率达９０％ 以上。经继代３ 年的胚性愈伤组织细胞的染色体倍性十分稳定，其染色体数及核型为２ｎ＝ ２４＝ １６ｍ （６ｓｃ）＋ ８ｓｍ ＋ ２Ｂ。这一结果与由实生苗根尖压片所得结果基本一致更多还原

【Abstract】 Embryogenic calli of Picea wilsonii Mast. cultured on modified 59 medium supplemented with 1 ppm 2,4 D and 1 ppm Kt still remained high reproductive potential after sequential monthly subcultures for 3 years. When the callus were transferred to 1/2 strength modification 59 medium with 1 ppm ABA, a lot of somatic embryos were produced after culturing about 3 monthes. The frequency of callus embryogenesis was above 90%. Chromosome counting showed that the chromosome ploidy of callus cells of P. wilsonii was stable in subcultures for 3 years. The chromosome number and karyotype formula is 2n=24=16m(6sc)+8sm+2B. This result agrees with that form root tip squash of seedling.更多还原