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菜豆下胚轴中的细胞分裂素结合蛋白
CYTOKININ BINDING PROTEINS FROM PHASEOLUS VULGARIS HYPOCOTYLS
【摘要】 用植物体内天然存在并具有很高活性的玉米素(t-Z)和异戊烯基腺苷(iPA)作为配基,分别与Sepharose-4B偶联,制成亲和吸附介质,分离、纯化菜豆(Phaseolus vulgaris)黄化幼苗下胚轴中的细胞分裂素结合蛋白。一种分子量为15.5 kD(简称ZBP),只含一个多肽链;另一种分子量为165 kD(简称IBP),含有两种亚基,分子量分别为43 kD和40 kD。对ZBP的结合活性进行了研究,发现ZBP与t-Z结合时的解离常数(Kd)为3.2×10- 7 m ol/L。经计算,每个ZBP分子只有一个t-Z结合位点
【Abstract】 t Zeatin (t Z) and isopentenyladenosine (iPA) occur naturally as highly active plant cell division regulators.t Z Sepherose 4B and iPA Sepherose 4B affinity column were constructed to isolate and purify the cytokinin binding proteins from etiolated hypocotyl of Phaseolus vulgaris.Two kinds of cytokinin binding proteins were obtained.One was 15 5 kD in molecular weight (named ZBP) with only one peptide.The other (named IBP), 165 kD in molecular weight, contained two different subunits (40 kD and 43 kD respectively).The binding activity of ZBP was tested and the dissociation constant (Kd) was determined to be 3 2×10 -7 mol/L.There was one binding site for t Z in each molecule of ZBP.
【Key words】 Cytokinin binding protein; Isopentenyladenosine; t Zeatin; Phaseolus vulgaris; Hypocotyl;
- 【文献出处】 植物学报 ,Acta Botanica Sinica , 编辑部邮箱 ,1994年11期
- 【分类号】Q946.1
- 【被引频次】1
- 【下载频次】46