【摘要】 天竺葵（Ｐｅｌａｒｇｏｎｉｕｍ ｈｏｒｔｏｒｕｍ Ｂａｉｌｅｙ）生殖细胞和精细胞在发育中始终存在质体和线粒体。在精细胞中，质体的体积大、数量多，具基质浓厚和在切面上多为环状的特点。线粒体在生殖细胞和精细胞中没有差异，体积较质体小得多，球形或杆状，边缘染色较深。在卵细胞中质体的含量比线粒体丰富，这两种细胞器的结构形态与精细胞的有明显的差异。细胞的质体多呈不规则的棒状和含淀粉粒。线粒体比精细胞的大２—３ 倍，许多为环状。ＤＮＡ 荧光的检测证明了在生殖细胞、精细胞和卵细胞中存在质体和线粒体类核。卵中的环状线粒体类核的形态在精细胞中是不存在的。本研究确定了雄性和雌性配子的质体和线粒体在结构形态上各具特点，可作为鉴别它们的标记，从合子中查明雄性质体和线粒体是否传递，以及在胚胎发育的早期雌雄亲本来源的细胞器的动态更多还原

【Abstract】 Electron microscopic investigation has demonstrated that plastids and mitochondria are conserved in the generative cell, sperm cells and egg cell of Pelargonium hortorum Bailey. The plastids in the generative cell which contain starch for a short period, gradually changed to proplastids during the maturation of generative cell. The plastids in the sperm cells are large and numerous the characteristics of dense matrix and an abundant endomembrane systems. These plastids always appear ringlike in cross section. In the generative cell and sperms, the spherical or rod shaped mitochondria are smaller than the plastids and remain unchanged during the development process from generative cell to sperm cells.DNA filaments are visualized in the transparent central zone of the mitochondria. In the egg cell, plastids are more abundant than mitochondria. The structures of the plastids and mitochondria are obviously different from those in the sperm cell. Most of the plastids are irregularly rod shaped and contain starch, the mitochondria are about 3 times larger than those in the sperm cells.Most of them are cup shaped as proved by successive sections. DNA epifluorescence study demonstrated that DNA nucleoids are present in both plastids and mitochondria of the egg, generative cell and sperm cells. In the sperm cells, there is no ringlike DNA nucleoid as is existed in the egg cell. This study has defined the characteristics of the plastids and mitochondria in both male and female gemates of P. hortorum. The results are essential contributions for further investigation of the biparental organelle transmission in the zygote and proembryo.更多还原