【摘要】 利用ＳＤＳ－ＰＡＧＥ及电子显微镜等技术比较分析和观察了两个大豆品种（Ｇｌｙｃｉｎｅ ｍ ａｘ（Ｌ．） Ｍｅｒｒ．）种子贮藏蛋白积累的差异。结果表明，在子叶发育过程中，蛋白含量高（４５％ ）的“公交８０５９－３”比蛋白含量低（３５％ ）的“ＧＤ－１５１５”蛋白含量高，且增长速率较快；蛋白质及７Ｓ和１１Ｓ亚基积累的时期较早，尤其是７Ｓ的β亚基。电镜观察表明“公交８０５９－３”蛋白质在液泡中的积累，前期与“ＧＤ１５１５”相似，中期以后的液泡中，蛋白体占整个细胞的比例均高于“ＧＤ１５１５”。这表明：大豆蛋白含量的差异有其生化及结构上的基础更多还原

【Abstract】 The difference in storage protein accumulation between two soybean was compared by SDS PAGE and electron microscopy. The results showed that the “Gongjiao 8059 3” (protein content 45%) was more rapid in protein accumulation rate and higher in protein content than that in “GD 1515”(protein content 35%) during cotyledon development. The subunits (7S and 11S) of the storage protein accumulated earlier in “Gongjiao 8059 3” than in “GD 1515”.Under electron microscope, the protein accumulated in vacuoles was similar between the two cultivars, but the protein density was higher and the volume of protein bodies was larger in cells after mid developing stage in “Gongjiao 8059 3”.These results showed that difference in protein contents may be based on biochemical and structural alterations.更多还原