节点文献
尿中83—1除草剂及其代谢产物的提取和分离方法的研究
Study on the Method for the Extraction and Separation of Ammonium Salt 2,4-dichloro-6-nitro-phenol and its Metabolites in Urine
【摘要】 本文报道了一种提取的分离尿中83—1除草剂及其代谢产物的HPLC方法:尿样酸化至pH3—4后,以氯仿—异丙醇（9+1）提取,提取物用Shin Pack ODSC18柱及甲醇—水（pH2.70）（65+35）进行HPLC分离,可良好地分离出83—1母体和4种代谢组分。本法重复10次测定,各组分的保留时间及峰高的相对标准差分别为0.16％～0.51％和1.2％～5.86％。如以保留时间定性、峰高或峰面积定量,本文所提供的提取分离方法还可用于83—1及其代谢产物的定性分析和定量测定。
【Abstract】 A method for the extraction and separation of ammonium salt 2,4-dichloro-6 -nitro-phenol(I)and its metabolites in urine with HPLC were reported. The urine sample was acidified to pH3-4 and extracted with chloroform and isopropanol (9 + 1) and then separated with Shin Pack ODS C18 column and CH3OH: H2O (PH2. 70) (65 + 35). The I and its four metabolites were well separated from each other. The RSD ofretention time and peak height of ten duplicates were 0. 16% - 0. 51% and 1. 2%~ 5. 86%, respectively. The proposed method can be applied to the qualitative and quantitative analysis of the I and its metabolites.
【Key words】 Urine Metabolites of ammonium salt 2； 4 -dichloro-6 - nitro - phenol High performance liquid chro-matography Extraction and separation；
- 【文献出处】 中国卫生检验杂志 ,Chinese Journal of Health Laboratory Technology , 编辑部邮箱 ,1994年01期
- 【分类号】R446.12
- 【被引频次】2
- 【下载频次】53