【摘要】 细粒棘球绦虫排泄分泌抗原研究──原头节排泄分泌抗原的反应原性朱兴全，窦兰清，史晓红，孙学勤，王晓华，牛炳亨（中国农业科学院兰州兽医研究所，甘肃７３００４６）笔者的初步研究已阐明，六钩蚴排泄分泌（ＥＳ）抗原具有较好的反应原性，纯化六钩蚴ＥＳ抗原可望能用...更多还原

【Abstract】 The excretory─secretory(ES)antigens of protoscolex,Echinococcus granulosus were pre-pared by in vitro cultivation of protoscolex for 14 days.The immune reactogenicity of protoscolex ES antigenswas evaluated by Enzyme─linked immunosorbent assay(ELISA) for the first time with the following sheepsera:The known positive sera with natural infection of E. granulosus,the known negative sera,sheep serawith experimentally infected E,granuIosus, sheep sera immunized with antigens,sheep sera from endemoepi-demic areas,sheep sera with natural infection of Fasciola hepatica,Cysticercus tenuicollis and Anaplasma sp.The micro─ELISA plates was coated with 0.2ml of antigens.The antigen concentration was 5μg/ml. The results show that the reactogenicity of protoscolex ES antigens was very well and was superior tothe crude extract of protoscolex.更多还原