【摘要】 用滤纸血以抗卫氏并殖吸虫囊蚴单克隆抗体Ｇ２Ｂ３间接双抗体夹心－ＥＬＩＳＡ检测感染卫氏并殖吸虫ＢＡＬＢ／Ｃ小鼠血中循环抗原（ＣＡｇ），结果显示：小鼠感染卫氏并殖吸虫囊蚴后第３天检出ＣＡｇ，其阳性率为３８％，第１５天起ＣＡｇ达高峰，阳性率１００％，２６～５６天ＣＡｇ维持高水平，第７１天ＣＡｇ滴度下降，以后维持在一定水平，说明滤纸血可以代替血清作ＣＡｇ检测。以抗卫氏并殖吸虫囊蚴单克隆抗体Ｇ２Ｂ３间接双抗体夹心－ＥＬＩＳＡ检测感染卫氏并殖吸虫小鼠血中ＣＡｇ时，具有简便，敏感和特异的优点，可试用于肺吸虫感染的早期诊断和流行病学调查。更多还原

【Abstract】 Observation on Kinetics of circulating antigen (CAg) in sera from mice infected with Paragonimus westermani was conducted with McAb to metacercaria G2B3 blood-absorbed filter paper and indirect double antibody sandwich-ELISA (McAb G2B3 DAS-ELISA ).The results revealed that CAg could be detected on third days,with positive rate 38%,The level of CAg elevated and reached the peak 15 days after infection. The CAg titre kept high level during 26-56 days from post-infection. Then CAg positive titre declined and maintained certain level on 71 days after infection thereafter. It suggested that it should be much simpler and easier to use blood-absorbed filter paper instead of sera for GAg detection.The results showed that McAb G2B3 DAS-ELISA with blood-absorbed filter paper was sensitive,specific,simpler and might be applied in serodiagnosis of patients with paragonimiasis and early diagnosis of patients with paragonimisis.更多还原