【摘要】 本研究应用薄层等电聚焦／ＳＤＳ──指数孔梯度ＰＡＧＥ双向电泳技术，对抗寒锻炼过程中冬小麦叶片组织可溶性蛋白质的变化进行了分析。从可溶性蛋白质含量电泳图谱的变化，可以看出，抗寒锻炼期间，京冬１号有９种可溶性蛋白质含量明显增加，肖农１２有１３种可溶性蛋白质含量明显增加。其中分子量为１ＫＤ、７ＫＤ（ＰＩ较低的一种）、１８ＫＤ（２种）、２１ＫＤ的５种蛋白质含量在２个品种中都与抗冻性呈正相关。表明寒胁迫过程中控制这些可溶性蛋白质合成的基因，在表达机制上发生了改变。更多还原

【Abstract】 Changes of soluble proteins in the leaf tissue of winter wheat were analyzed by using 2-dimentional electrophoretic techniques of ultrothin gel IEF/SDS-concave exponential gradiant PAGE during cold hardening and dehardening.From the 2-D eletrophoretic patterns of soluble proteins,We found that there were 9 proteins,of which the content obviously increased in the leaf tissue of a wheat cultivar Jingdong No.1 during cold hardening while there were 13 proteins with increased content in another wheat cultivar Xiao nong 12.Among these,the content of 5 proteins with clear molecular weight 1KD,7KD (the one with lower PI),18KD(2 with different PI).21KD was positively correlated with the development of freezing resistance in two cultivars.更多还原