【摘要】 为研究Ｔ细胞分化发育的过程，在体外建立了７株小鼠胸腺基质细胞系，应用这些小鼠胸腺基质细胞系及其培养上清在体外与经过分离的裸鼠骨髓干细胞共育７２小时后，用双荧光抗体染色，分析共育后的裸鼠骨髓细胞，发现有部分细胞分化为ＣＤ￣（３＋）ＴＣＲαβ￣－，ＣＤ３￣（３＋）·ＴＣＲαβ和ＣＤ３￣－ＴＣＲαβ￣＋细胞亚群，不同的胞腺基质细胞系和培养上清的诱导作用亦有差别。更多还原

【Abstract】 Seven mice thymic stromal cell(MTSC ) Iines,derived from thymus of Balb/c mice,estab-lished in our Iaboratory. To study the roles of these mice thymic stromal cell Iines on the differ-entiation of bone marrow stem cells,we coculture these MTSCS and MTSC- supertants withnucle mice fresh bone marrow cells seperated by 1. 060 Ficoll-Urografin. The phenotypes of vi-able bone marrow cells after culture with MTSCS or MTSC -SNS for 3 day, stained by PE-anti-TCRαβ McAb and FITC-anti-CD3 McAb, were analysed on flow cytometry(FACS).Theresult showed that MTSCS and MTSC-SNS can induce nude mice bone marrow cells expressingCD3 /TCRαβmolecules in different degree. Provide a model for further studying T cells differen-tiation in vitro.更多还原