【摘要】 观察了小鼠接种高分泌ＩＬ－４的黑色素瘤细胞后体内免疫效应细胞（包括ＣＴＬ、ＮＫ细胞及ＬＡＫ细胞）抗肿瘤活性及其分泌的细胞因子（包括ＩＬ－２、ＩＦＮ－ｒ、ＴＮＦ及ＧＭ－ＣＳＦ）水平的变化，荷瘤后第１５天小鼠脾细胞ＮＫ活性及经诱导后的ＬＡＫ活性有所降低，而经诱导后的ＣＴＬ杀伤活性显著升高，荷瘤小鼠腹腔内巨噬细胞杀伤活性及其分泌的ＩＬ－１水平也明显升高；在荷瘤后第４天及第１２天出现两个高峰，实验结果表明，高分泌ＩＬ－４的黑色素瘤细胞体内生长受抑制是因为ＩＬ－４基因的导入及ＩＬ－４的分泌使体内ＣＴＬ及巨噬细胞杀伤活性提高，因而使机体抗肿瘤免疫功能得以增强。更多还原

【Abstract】 he in vivo induction of killer cells and cvtokines was observed in the mice inoculated withtumor cells engineered to secrete IL-4 in this study. The NK and the LAK activity induced fromthe splenocytes of the tumor-bearing mice decreased slightly but the cytotoxicity of CTL in-creased significantly.The levels of IL-2‘and IFN-γ induced from the splenocytes decreased butthe levels of TNF and GM-CSF increased. The results denmonstrated that the induction of somekinds of killer cells and cytokies are one of reseans for the in vivo growth inhibition of tumorcells transfected with IL-4 gene.更多还原