【摘要】 采用膜上斑点免疫结合测试技术（Ｄｏｔ－ＩｍｍｕｎｏｂｉｎｄｉｎｇＡｓｓａｙ，ＤＩＢＡ）快速检测灰稻虱（ＬａｏｄｅｌｐｈａｘｓｔｒｉａｔｉｌｂｕｓＦａｌｌｅｎ）的毒率。试验得出用含２％牛血清白蛋白（ＢＳＡ）、２％ＰＶＰ、０．０５％Ｔｗｅｅｎ２０的０．０２ｍｏｌ／ＬＰＢＳ作封缓覆冲液、封闭３０ｍｉｎ后使抗原（Ａｇ）与其相应抗体（Ａｂ）和抗体与酶标记Ａ蛋白（ＨＲＰ－ＳＰＡ）各反应３０ｍｉｎ，检测效果较佳，灵敏度比相应的ＥＬＩＳＡ提高４倍。更多还原

【Abstract】 The scverity of riee stripe discase is affectal directiy by the pcrcentage of viruliferousLaodelphax strtoiellus in the field. In order to forccast the disease correctly. the percentage ofviruliferous L. strmtellus must be detected quickly and precisely. The Dotlmmunobinding Assay(DIBA) for rapid detectog the viruses in L. strmiellus was siudled it was found that the optimal bufferis 0.02mol/ L PBS containing bovine serum albunmin 2% BSA , 2%. PVP and 0.05% Tween 20, theoptimal blocking and reaction time are both 30 nuinutes. Four timee of detection senntivity are increasedby DIBA than by ELISA.更多还原