【摘要】 应用聚合酶链反应（ＰＣＲ）技术，并配合限制片段长短的多态现象（ＲＦＬＰ）及ＣＡ重复顺序多态性分析，对１５例乳腺癌病人３号染色体短臂３ｐ２４，７号染色体长臂７ｑ３１，１６号染色体长臂１６ｑ２４，３和１７号染色体短臂１７ｐ１３．１杂合缺失（ＬＯＨ）进行了测定。在上述位点分别发现３６％、３６％、２／４和５／９的信息个体呈现ＬＯＨ。其中３ｐ２４和７ｑ３１位点的ＬＯＨ大多发生在临床ＩＩ、ＩＩＩ期、淋巴转移阳性和雌激素受体阴性的肿瘤中。提示３ｐ２４和７ｑ３１位点ＬＯＨ的测定有可能成为乳腺癌病人预后评价的指标。此外，上述四个位点所呈现的较高的ＬＯＨ阳性率也提示乳腺癌抑癌基因在这些位点存在的可能性。更多还原

【Abstract】 he loss of heterozygosity (LOH) at chromosomes 3p24, 7q31, 1 6q24.3 and 17p13. 1 was measuredin 15 patients with breast cancer by means ofpolymerase chain reaction (PCR)-restriction fragmentlength polymorphisms (RFLP) or PCR-CA repeatanalysis. In 36%, 36%, 50% and 56% of the informa-tive cases, LOH was detected at 3p24, 7q31, 16q24.3and 17p1 3.1 loci, respoctively. All the LOH at 3p24 or7q31 happened in stage 11 or III, 67% of the LOH at3p24 or 7q31 happened in the cases with lymph node metastasis, and 67% of the LOH at 3p24or 7q31 were found in estrogen receptor negative pa-tients. This finding shows that the measurement ofLOH at 3p24 and 7q31 may be an useful predictingmarker for the prognosis of patients with breastcancer. The high percent of LOH at these four locimay also indicate the possibility of the existence oftumor suppressor genes for breast cancer.更多还原