【摘要】 用大肠杆菌ＰＱ３７和ＰＱ３５，分别以４－硝基喹啉－Ｎ－氧化物（４ＮＱＯ）和丝裂霉素Ｃ（ＭＭＣ）微量持续和／或脉冲诱导模式测定了１５种中药水提物、蔬菜汁和化学品溶液对依ｌｅｘｓｆｉ－ＳＯＳ反应的影响。结果表明，黄精、诃子、首乌、女贞子、浙贝母、带蒂菱殳水提物，韭菜汁以及５－氟尿嘧啶、鞣酸和大蒜素溶液对４ＮＱＯ持续和脉冲诱导的ＳＯＳ反应有线性抑制作用，提示该作用发生在ＰＱ３７菌细胞内和外；其中诃子、女贞子、浙贝母、带蒂菱殳水提物、韭菜汁、５－氟尿嘧啶、鞣酸溶液还能在ＰＱ３５胞内抑制ＭＭＣ诱导的ＳＯＳ反应。灰树花兼有在ＰＱ３７外或ＰＱ３５内抑制４ＮＱＯ或ＭＭＣ诱导的ＳＯＳ反应。而生山楂、白芷、麦冬、５－氟脱氧尿苷仅能在ＰＱ３７外抑制４ＮＱＯ诱导的ＳＯＳ反应。更多还原

【Abstract】 ffects of 15 kinds of herbal drugs,vegetables and chemicals on lex-dependent sfi-SOS response were determined by micropersistent and/or pulse models induced by 4-Nitroquinoline-N-oxide(4NQO) and Mitomycin C(MMC)in Escherichia coli(E. coli)PQ37 and PQ35,respectively Results showed the water extract of Rhizoma Polygonati(RP),Fructus Chebulae(FC),Radix Polygoni Multiflori(RPM),Fructus Ligustri Lucidi(FLL),Bulbus Fritillariae Thunbergii(BFT),shell of water chestnut with a pedicle,Chinese chives juice,and solutions of 5-Fluorouracil,Tannic acid and garlicin could inhibit SOS responses with a dose-response relationship and suggested the inhibitory effects took place both inside and outside E.coli cells.Water extract of FC,FLL,BFT,shell of water chestnut with apedicle,Chinese chives juice and solution of 5-Fluorouracil and Tannic acid could intracellularly in-hibit SOS responses induced by MMC in E.coli PQ35,and acetone extract of Grifola Frondosa(GF)could extracellularly inhibit SOS responses in E.coli PQ37 and intracellularly in PQ35 induced by 4NQO or MMC.Water extract of raw hawthorn,Radix Angelicae Duhuricae(RAD),Radix Ophiopogonis(RO),and 5-Fluorodeoxyuridine could extraeellularly inhibit SOS responses induced by 4NQO in E coli PQ37.The possible mechanisms of intracellular inhibition and antidamage repair were discussed in the paper.更多还原